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If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Spermatozoa interact with oviduct secretions before fertilization in vivo but the molecular players of this dialog and underlying dynamics remain largely unknown.
Our objectives were to identify an exhaustive list of sperm-interacting proteins SIPs in the bovine oviduct fluid and to evaluate the impact of the oviduct anatomical region isthmus vs. Pools of oviduct fluid OF from the pre-ovulatory ampulla, pre-ovulatory isthmus, post-ovulatory ampulla, and post-ovulatory isthmus in the side of ovulation were collected from the slaughterhouse.
Frozen-thawed bull sperm were incubated with OF or phosphate-buffered saline control for 60 min at A quantitative comparison between proteins identified in sperm and OF samples and proteins, respectively allowed for the identification of SIPs. The highest number were found in the pre-ovulatory isthmus, i. Functional analysis of SIPs predicted roles in cell response to stress, regulation of cell motility, fertilization, and early embryo development.
This study provides a comprehensive list of SIPs in the bovine oviduct and evidences dynamic spatio-temporal changes in sperm-oviduct interactions around ovulation time.
