Official websites use. Share sensitive information only on official, secure websites. Correspondence to: Richard J. Bryant, richard.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Epithelial to mesenchymal transition EMT of cancer cells involves loss of epithelial polarity and adhesiveness, and gain of invasive and migratory mesenchymal behaviours.
EMT occurs in prostate cancer PCa but it is unknown whether this is in specific areas of primary tumours. We examined whether any of eleven EMT-related proteins have altered expression or subcellular localisation within the extraprostatic extension component of locally advanced PCa compared with other localisations, and whether similar changes may occur in in vitro organotypic PCa cell cultures and in vivo PCa models. These results suggest that changes in EMT protein expression can be observed in the extraprostatic extension component of locally invasive PCa.
The biology of some of these changes in protein expression may be studied in certain in vitro and in vivo PCa models. Keywords: epithelial to mesenchymal transition, extraprostatic prostate cancer, immunohistochemistry, in vitro organotypic cell culture, in vivo mouse prostate cancer model, Pathology Section. It is estimated that , men in the United States will be diagnosed with prostate cancer PCa during and 27, men will die from this malignancy [ 1 ], usually from metastatic disease.
Within the TNM classification of PCa, the pT3a stage of this malignancy describes extraprostatic extension of the tumour beyond the prostatic capsule. Extraprostatic extension of PCa is associated with recurrence and metastasis following radical treatment [ 2 - 5 ].
